Recruitment of the adaptor protein Grb2 to EGFR tetramers.

Using the same background subtraction procedure, a lifetime of 2.5 ± 0.03 ns was obtained for EGFR–eGFP in the presence of EGF and Grb2–mRFP. A Student’s t test revealed that the difference in apparent lifetimes (2.85 vs 2.50 ns) was highly significant ( p < 0.0001, standard error about the mean 0.03 ns, sample size = 50).

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