Quantitative real-time reverse transcription-polymerase chain reaction (qPCR) The gene expression of the early bone-specific marker Runx2 ( Figure 6(a) ) examined over 28 days in test sample HA and monetite scaffolds seeded with hMSCs in non-osteogenic-conditioned medium showed that the monetite scaffold demonstrated an increasing trend that was not statistically significant over the 28-day time period while HA was reduced at day 14 but increased by day 28.
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In vitro osteoinductive potential of porous monetite for bone tissue engineering.
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