High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics.

45 With respect to the glycolytic pathway downstream of glycerate 1,3 bisphosphate (1,3BPGA), phosphoenolpyruvate carboxykinase (PEPCK), malate dehydrogenase (MD), and NADH-dependent fumarate reductase (FR) are all clearly present in the group (iii) proteins, but fumarate hydratase (FH) is, like enolase (ENO) and malic enzyme (ME), a group (i) protein, suggesting that all are located without the glycosome. This may be significant because the presence of FH in the glycosomes as well as in the cytosol has been previously suggested. 46 The absence of FH in the glycosomes would mean that the transmembrane transport of malate and fumarate would be essential for the production of glycosomal succinate, required for NAD + /NADH balance inside the procyclic glycosome.

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