DNA binding specificities of the long zinc-finger recombination protein PRDM9.

Its best alignment to the predicted site had eight strong matches (Figure 3G ), which is likely to be significant ( P = 0.00098).

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Using the χ 2 test, the probability that the number of nucleotide matches between the three sites (scored as 0, 2, or 3 matches at each position) exceeds chance was 0.048 (barely significant), and this significance entirely disappeared when we considered only the 17 positions where the mutation analysis (see below) indicated the strongest evidence for nucleotide specificity.

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