marginally significantP = 0.05
In the long-term assay, the pupation rate gradually decreased with the increase of pollen density; the decrease was marginally significant with control pollen ( F = 3.12, df = 17, P = 0.05), but was significant with both Cry1C pollen ( F = 6.58, df = 17, P = 0.004) and Cry2A pollen ( F = 6.77, df = 17, P = 0.004).