Identification and Quantitation of Coding Variants and Isoforms of Pulmonary Surfactant Protein A.

Here, we established, for the first time, a facile assay for targeted quantitation of allelic variants at position 223 of SP-A, as well as relative levels of SP-A2 versus total SP-A, using as little as 1 mL of bronchoalveolar lavage fluid. While there was no significant difference in relative SP-A2 as a function of 223 genotype, there was a slight trend toward lower SP-A2 levels versus total SP-A in the Lys223/Lys223 versus Gln223/Lys223 genotype.

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