IVT-seq reveals extreme bias in RNA sequencing.

Genome Biol · 2014 · PMC4197826 · PMID 24981968

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highly significantP <2.2e -16actually significant

Furthermore, we saw a slight but highly significant correlation (Pearson R 2 = 0.308452; P <2.2e -16 ) between a transcript sequence’s similarity to rRNA and the magnitude of the difference in coverage between the no selection and rRNA-depleted libraries (Additional file 11 : Figure S9 and Additional file 12 ).

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