highly significantP <0.001
In a previous study on the LiTOX strain, we have validated the reliability of mRNA sequencing pooled data for inferring population allelic frequencies on 269 SNP: the correlation between allelic frequencies obtained from pooled mRNA sequencing (180 pooled larvae) and individual genotypes (N = 28 individuals) obtained using a DNA Illumina GoldenGate array was highly significant (P <0.001, r =0.85) [ 51 ].