highly significantp<0.001
Furthermore, sperm viability and organelle functionality showed a decrease after thawing, displaying highly significant differences between 0 and 2 hours post-thawing: YOPRO-1-/PI-, t(32) = 11.47, p<0.001; MT+, t(32) = 6.26, p<0.001; and PNA-, t(32) = 11.18, p<0.001 ( Table 2 ).