Enhanced neutrophil extracellular trap generation in rheumatoid arthritis: analysis of underlying signal transduction pathways and potential diagnostic utility.

Accounting for variances in PMN counts, the difference between the healthy control and RA subjects remained highly significant in an analysis of covariance (ANCOVA) of the nucleosome assay ( P < 0.001).

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Of interest is that no significant difference was found in this value regardless of whether the RA cases were ACPA positive or not (Figure 5 G), although a slight trend for serum nucleosome levels was higher in ACPA-positive cases than in ACPA-negative cases (Figure 5 H).

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