highly significantp < 0.01
The mean quality score for all bases from bisulfite converted libraries (N = 4) across each of the 5 lanes was (1% PhiX: 37.58 ± 0.03, 10% PhiX: 37.56 + 0.08, 20% PhiX: 37.44 ± 0.1, 30% PhiX: 37.18 ± 0.07, 50% PhiX: 36.42 ± 0.07), which corresponds to a linear, highly significant (p < 0.01 for all comparisons) decrease in quality as PhiX spike-in concentration increased; Importantly, all of these quality scores are excellent (mean phred score >35).