Exploring features and function of Ss-riok-3, an enigmatic kinase gene from Strongyloides stercoralis.

Ss-riok-3 -specific transcript abundances were calculated as fragments per kilobase of coding exon per million fragments mapped (FPKM), and significant differences in FPKM values between developmental stages were determined using Cufflinks v.2.0.2 ( http://cufflinks.cbcb.umd.edu/ ) [ 35 , 38 - 40 ]; p -values <0.05 were considered statistically significant, and p -values <0.001 were considered statistically highly significant.

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There was a generally an increasing trend in Ss-riok-3 transcript abundance during development from PFL L1 to iL3 in the external environment, and in the further development within the host to the L3+ and then to the P Female.

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