Genomic identification and characterization of the pseudoautosomal region in highly differentiated avian sex chromosomes.

To identify the PAR, we focused on SNPs heterozygous in both males and females in a pool of unmapped markers that were not linked to any of the 33 autosomal linkage groups 33 . Seven of these previously unlinked markers from three small scaffolds (N00298, three markers in 436.0 kb; N00378, three markers in 182.2 kb; N02597, one marker in 2.3 kb) showed highly significant two-point linkage in both male and female meioses to several markers located close to one end of the Z chromosome linkage map and to each other ( Supplementary Table 1 ; Supplementary Fig. 1 ).

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