Human-caused habitat fragmentation can drive rapid divergence of male genitalia.

Male Gambusia from fragmented tidal creeks exhibited a marginally nonsignificant trend toward possessing smaller gonopodia than counterparts in unfragmented sites (Table 1 ).

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Number of spines on the gonopodial distal tip scaled positively with distal-tip size and exhibited a nonsignificant trend toward scaling negatively with relative gonopodium size (Table 5 ).

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Our study revealed no differences in gonopodial meristics between fragmentation regimes other than a weak trend for fewer serrae in fragmented tidal creeks.

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