We screened for highly significant BLASTX hits (max E-value 1e-10) using the NCBI BLAST package (version 2.2.25) and identified the top hit (lowest E-value, best scoring HSP covers minimum 25% of the hit) using custom PERL scripts.
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Inferring bona fide transfrags in RNA-Seq derived-transcriptome assemblies of non-model organisms.
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