Pre-amplification in the context of high-throughput qPCR gene expression experiment.

BMC Mol Biol · 2015 · PMC4365555 · PMID 25888347

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highly significantp < 0.001actually significant

We show that the number of Cycles had a highly significant effect on overall likelihood of ‘success’ (p < 0.001) if tested for all Genes and Concentration together.

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