Combined effect of a mixture of tetracycline, acid, and detergent, and nisin against Enterococcus faecalis and Actinomyces viscosus biofilms.

Compared with the negative control where a gradual decline in the mean bacterial counts was observed between 5 and 15 mins, a highly significant reduction ( p =0.000) in mean bacterial counts was observed for E. faecalis (77.3+13.6) and A. viscosus (39.6+12.6) after 5 min exposure to MTADN approaching to almost no growth after 10 min and 15 min exposure.

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