Pre-analytical sample quality: metabolite ratios as an intrinsic marker for prolonged room temperature exposure of serum samples.

All but one of the 9 lysophosphatidylcholines (lysoPC(18:2)) showed highly significant changes due to pre-storage conditions, with p-values for the time coefficient, the temperature coefficient and the interaction between 10 –5 and 10 –53 ; all of these 8 lysophosphatidylcholine concentrations increased clearly at RT ( Fig 2 ).

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Glutamine is the only amino acid with a decreasing trend, though not significant as a single metabolite.

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