Production of a new non-specific nuclease from <i>Yersinia enterocolitica</i> subsp. <i>palearctica</i>: optimization of induction conditions using response surface methodology.

erature ( B ), IPTG concentration ( C ) and induction time ( D ). In order to estimate the optimal enzyme activity, the full quadratic second-order polynomial equation was fitted by applying multiple regression analysis on the experimental data ( Table 2 ): (2) As shown in Table 3 , the model was highly significant ( p < 0.01).

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