A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

No correlation was found for the monomer signal ( S4 Fig ), however, a slight trend may be discerned in the dimer fraction possibly decreasing by about 2% per year ( S5 Fig ).

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(Three instruments were equipped with a 675 nm laser, as opposed to the currently used 655 nm laser, which causes only a ≈0.28% calculated difference in the value of dn/dc , based on [ 40 ], which was neglected in the consideration of signal magnitudes.) Thus, an instrument-dependent error of at least ≈3–4% in concentration should be considered when determining protein concentrations based on an assumed refractive index increment and given fringe shift data (in addition to variations in the protein refractive index increments, which may be significant especially for smaller proteins [ 41 ]).

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