highly significantP < 0.001
The effect of diet type on TDF was also highly significant for the other Tr-AAs, such as alanine ( F 2,14 - 142.97, P < 0.001), valine ( F 2,14 - 118.70, P < 0.001), leucine ( F 2,14 - 189.65, P < 0.001), and isoleucine ( F 2,14 - 68.67, P < 0.001).