When the cells were treated one time with αAM or αAMR, we observed a significant increase in Trypan Blue-BSA permeability compared to control cells, which was highly significant after a delay of about 6 h and started to decrease by 24 h to reach the control values by 30 h of incubation (Figure 4 ).
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Adrenomedullin blockade induces regression of tumor neovessels through interference with vascular endothelial-cadherin signalling.
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