TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR.

PLoS One · 2015 · PMC4501803 · PMID 26172450

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highly significantno p-value reported

Other two combinations of MRQ primer pairs, MRQ2/MRQ5 and MRQ2/MRQ4, also exhibited highly significant correlations with BRQ1/BRQ2 (R = 0.89 and R = 0.87, respectively) in terms of validating the quality of the 13 tested cDNA samples.

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