Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor.

It may be significant that an rRNA nucleotide near the 5′ end of the 16S rRNA intercalates within the S4–S5 interface, connecting the S4–S5 interface with the structure of the 5′ end and central pseudoknot, implicating these structures in translational accuracy as well.

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