A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo.

Double-labelling with antibodies to Smo and to acetylated tubulin (which marks stable microtubules found in cilia) revealed a highly significant accumulation of Smo in the cilia of NIH-3T3 cells upon addition of ShhN ( Fig. 6 B,G; χ 2 =61.19, d.f.=1, n =157 cells, P =5.16×10 −15 ) whereas, in the absence of ShhN, cilia are largely devoid of Smo ( Fig. 6 A,G) ( Rohatgi et al., 2007 ).

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