A Comparison between Transcriptome Sequencing and 16S Metagenomics for Detection of Bacterial Pathogens in Wildlife.

PLoS Negl Trop Dis · 2015 · PMC4540314 · PMID 26284930

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Correlations between zoonotic bacterial read number and their prevalence were weak using both RNA-Seq (R 2 = 0.053, P = 0.069; Fig 2 ) and 16S-454 approaches (R 2 = 0.088, P = 0.027; Fig 2 ), whilst the correlation was positive and highly significant for the 16S-MiSeq methodology (R 2 = 0.763, P <0.001; Fig 2 ).

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