High Efficacy but Low Potency of δ-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments.

In accordance with the data obtained in analysis of LDM isolated at 0°C, comparison of the dose-response curves of DADLE-stimulated [ 35 S]GTPγS binding in plasma membranes exposed to 0.006% Brij-58 at 30°C indicated a highly significant decrease in potency of agonist response: EC 50 value was increased from 24 ± 5 nM (no detergent) to 120 ± 10 nM in PM exposed to 0.006% Brij-58 ( Fig 13 , S13 Table ).

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