Direct sequencing of human gut virome fractions obtained by flow cytometry.

Front Microbiol · 2015 · PMC4568480 · PMID 26441889

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an increasing trendno p-value reported

This requirement resulted in an increasing trend to amplify extracted DNA before sequencing, e.g., by non-specific PCR amplification of DNA fragments ( Stang et al., 2005 ; Ambrose and Clewley, 2006 ; Solonenko et al., 2013 ) or whole genome amplification (WGA) ( Breitbart et al., 2008 ; Pérez-Brocal et al., 2013 ; García-López et al., 2014 ).

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