highly significantP < 0.01
Also, large and highly significant levels of differentiation (F ST = 0.22, P < 0.01) were found among Italian populations sampled in 2009 ( S2 Table , Table 3 ) despite a relatively short distance of 50 km separating them (i.e., Friuli Venezia Giulia [Venezia district] and Cordenons [Pordenone district]).