Bearing these issues in mind, being stringent on base quality thresholds and only using contigs with highly significant BLAST hits (an e-value of ≤1e −4 is commonly used throughout the literature [ 22 ]) to previously published, putatively homologous sequences (e.g. those found in NCBI non-redundant and Swiss-Prot databases) will help to ensure high confidence in the resultant assembly.
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Transcriptome sequencing, annotation and polymorphism detection in the hop bush, Dodonaea viscosa.
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