BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis.

Deletion of h+58 phenocopied deletion of the composite enhancer and deletion of h+55 had moderate effect (while deletion of h+62 showed a nonsignificant trend towards a modest effect), consistent with the magnitude of top-scoring and colocalizing sgRNAs from the screen ( Fig. 2a, c-e ).

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