Spatial and temporal variation at major histocompatibility complex class IIB genes in the endangered Blakiston's fish owl.

A highly significant excess of non-synonymous over synonymous substitutions was observed for codons in the putative peptide-binding regions (PBR), whereas synonymous substitutions were more frequent than non-synonymous substitutions in non-PBR codons (Table 2 ).

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An AMOVA also detected weak but partially significant genetic differences among periods whitin populations (Table 3 ).

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