Differences were considered significant if P was ≤0.05 and highly significant if P was ≤0.01. ΔΔCt was calculated as follows: (Ct target –Ct actin ) the gonad stage −(Ct target −Ct actin ) the stage II ovary (Qu et al. 2014 ).
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Cloning and transcriptional expression of a novel gene during sex inversion of the rice field eel (Monopterus albus).
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