The greatest, and highly significant, difference was found for transcripts associated with immune system processes, which constituted only 1.8 % of genes represented in both ventral feathers and organs as compared to 12.3 % of genes represented in both dorsal feathers and organs . qPCR analysis In order to explore these expression differences further, we conducted qPCR on 20 samples comprising 11 ventral and 9 dorsal feathers sampled from 7 dark, 2 intermediate and 8 light buzzards (see Additional file 1 : Table S1).
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De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite.
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