AnABlast: a new in silico strategy for the genome-wide search of novel genes and fossil regions.

BLAST analysis efficiently identifies homologous sequences through highly significant alignments. 3 However, because a high frequency of low significant alignments may reveal stretches of ancestral regions, 21 , 22 we used a low-stringency BLAST strategy to include alignments beyond a significant similarity, a key step in our strategy to identify putative coding sequences with a biological and/or evolutionary significance (Fig. 1 ).

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