Although the negative- strand of TMV(62A+UPD) viral RNAs were detected from the transfected protoplasts by qRT-PCR, it showed a decreasing trend and the amount was significantly lower than that of TMV, TMV(24A+UPD) and TMV(42A+UPD).
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TMV mutants with poly(A) tracts of different lengths demonstrate structural variations in 3'UTR affecting viral RNAs accumulation and symptom expression.
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