2A ) such that hemolymph [Na + ] (measured prior to cold exposure) had a highly significant negative relationship with cold tolerance (shown as LTe 50 , but relationships were significant regardless of the tolerance measure used; R 2 = 0.99, df = 3, P < 0.001; Fig. 2A insert).
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The capacity to maintain ion and water homeostasis underlies interspecific variation in Drosophila cold tolerance.
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