Estimation of allele-specific Ace-1 duplication in insecticide-resistant Anopheles mosquitoes from West Africa.

Malar J · 2015 · PMC4683970 · PMID 26682913

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highly significantP = 4 × 10 −8actually significant

There was a strong and highly significant difference in copy number (estimated from the qRTPCR) among the groups identified by TaqMan and ddPCR (ANOVA: F 4,38 = 18.4, P = 4 × 10 −8 ) with a progressive increase from a single-copy average for susceptible homozygotes (Gn), suggesting that all or most are unduplicated, to resistant homozygotes (Sn) which averaged in excess of four gene copies (Fig. 6 ).

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