A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling.

BMC Genomics · 2016 · PMC4712552 · PMID 26763898

Europe PMC DOI

hedged sentences

0.1100

closest p · 2.2× alpha

0.2200

boldest claim

The sentences

marginally significantp =0.11not close (p > 0.1)

The only consistent effect observed on error rate was a marginally significant increase associated with more PCR cycles ( p =0.11 - see Fig. 5 a ).

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marginal significancep =0.22not close (p > 0.1)

Using 1 ng of template reduced the proportion of chimeric reads compared to 10 ng but only with marginal significance ( p =0.22) (see Fig. 5 b ).

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highly significantno p-value reported

In both cases there is a highly significant correlation between the two ratios and a slope that is nearly one for a regression forced through the origin.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.