Real-time quantitative RT-PCR using RNA isolated from wild-type and the ether lipid-deficient mutants showed a highly significant decrease in expression for fat-5 and fat-7 in fard-1 , ads-1 , and acl-7 mutants compared with wild-type, while gene expression changes in ads-1 mutants compared with wild-type for the genes fat-6 , elo-2 , and pod-2 showed a similar trend, as observed with the semi-quantitative RT-PCR results, but it was not significant ( Fig. 4D ).
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A Caenorhabditis elegans model for ether lipid biosynthesis and function.
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