CRISPRi-Manipulation of Genetic Code Expansion via RF1 for Reassignment of Amber Codon in Bacteria.

The results of the quantitation of RF1 repressions versus GFP fluorescence and cell growth indicated an increasing trend of UAA incorporation following the initial RF1 repression, as shown in sgRNA7 versus sgRNA9 versus sgRNA0 ( Fig. 3A ); a maximum of 20-fold improvement in UAA incorporation was obtained ( Fig. 3B ).

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