microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration.

Transfection of murine MG cells with a pLightSwitch TREM2-3’-UTR-luciferase reporter vector Functional validation of a miRNA-34a-TREM2–3’UTR interaction in MG cells is shown in Fig 4 ; briefly, transfection of MG cells with a pLightSwitch TREM2-3’UTR-luciferase reporter (containing the entire 299 nt TREM2 mRNA 3’UTR; showed no significant effects on relative luciferase signal strength in controls, however, in the presence of a miRNA-34a mimic TREM2-3’UTR-luciferase signals were quenched ~4.5-fold of control and the results were highly significant ( Fig 4 ).

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