A pipeline to quantify serum and cerebrospinal fluid microRNAs for diagnosis and detection of relapse in paediatric malignant germ-cell tumours.

A highly significant correlation was observed between the Cq values obtained in the initial QC ( Figure 1 , box B) and the miRNA quantification steps ( Figure 1 , box C), for both cel–miR–39–3p and miR–30b–5p ( R 2 =0.937, P <0.0001 and R 2 =0.867, P <0.0001, respectively; Supplementary Figure S1E ; Figure 1 , box D).

also in 132,142 other papers