highly significantP < 0.001
Molecular mapping of QTLs associated with seed-Fe and Zn concentrations A highly significant difference (P < 0.001) of seed-Fe (51.3 ± 5.0, 40.3–67.0 ppm with 81% H 2 ) and Zn (36.0 ± 3.8, 27.9–48.5 ppm with 83% H 2 ) concentrations in two parental accessions and 277 individuals of a RIL mapping population [ICC 4958 (Fe: 43.0 ± 2.9 and Zn: 30.2 ± 2.5 ppm) × ICC 8261 (Fe: 64.8 ± 3.3 and Zn: 42.0 ± 2.7 ppm)] across two years based on ANOVA was observed ( Table S3 , Fig. 3A,B ).