Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates.

BMC Bioinformatics · 2016 · PMC4827196 · PMID 27067838

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highly significantno p-value reported

The tests for a vanishing Δ Δ C q are all highly significant with comparable 95 % CIs.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.