Effect of Different Broad Waveband Lights on Membrane Lipids of a Cyanobacterium, Synechococcus sp., as Determined by UPLC-QToF-MS and Vibrational Spectroscopy.

All the cultures had a reduction ( p = 0.038, two-way ANOVA) in the SQDG (18:3/16:0) content after 48 h of growth as compared to the content after 24 h of growth, with an apparent trend of the content in the B culture being higher than in the other cultures (although only significantly different between the B and G cultures as indicated above).

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Because there is a slight trend towards increased peaking at 1282 cm −1 in the G and B cells from the peaking at 1272 cm −1 in the W cells, with an intermediate situation in R cells, this band may correspond to the tetrapyrrole ring of phycobilins as the A 630 /A 680 ratio suggests a similar situation.

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In general, absorbance intensity at wavenumbers between 1700 and 1340 cm −1 exhibited a decreasing trend B > G > R > W, with defined bands peaking at about 1643, 1545, 1453 and 1401 cm −1 although the peak at 1453 cm −1 progressively disappeared from B to W.

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