Uncovering the Relationship between Sulphation Patterns and Conformation of Iduronic Acid in Heparan Sulphate.

In the IdoA-containing series of hexasaccharides ( 5 – 8 ), the NOESY analysis indicated a decreasing trend in the ratio of H2-H5/H4-H5 NOE signals, suggesting that the 2 S O conformer becomes less abundant as the surrounding sulphation level increased.

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The ratio of H2-H5/H4-H5 NOE signals among 1 – 4 exhibited an increasing trend as the level of sulphation elevated ( Table 1 ), consistent with the conclusion from the J analysis.

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