Microsatellite Length Scoring by Single Molecule Real Time Sequencing - Effects of Sequence Structure and PCR Regime.

In each comparison we found that pre-sequencing PCR increased errors in a highly significant way (p<<0.001 for all comparisons, unpaired Wilcoxon signed rank test).

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The restriction digest controls form high leverage points when modelling this as a function of the number of PCR cycles, leading to significant or marginally significant models ( S25A–S25C Fig ) (30xCA: p = 0.058, 20xATCT: p<0.001, 10xATCT: p<0.001; GAM).

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