Astrocyte morphology is confined by cortical functional boundaries in mammals ranging from mice to human.

However, a highly significant change was found in the number of crossings when comparing the two sub-laminar areas at similar distances from the borders; 27.2 ± 1.15 vs 13.3 ± 0.54, t 66 = 22.39, p<0.001, 50 μm, and 28.5 ± 1.07 vs 12.4 ± 1.37, t 68 = 18.44, p<0.001, 200 μm from the border, indicating an abrupt change in astrocyte crossing at the sublaminar border.

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In contrast, there was only a slight trend of reduced crossings of blood vessels at barrel's border (2.64 ± 0.18 vs 2.97 ± 0.18. 95% confidence intervals, 2.47 to 2.81 and 2.77 to 3.17) which did not reach significance (t 156 = 1.28, p=0.2; Figure 2F , right).

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