highly significantP < 0.001
After 42 cycles, however, the selection × assay environment interaction was highly significant in the linear pathway ( F (9, 368) = 6.6, P < 0.001), the branched pathway ( F (9, 368) = 6.3, P < 0.001), and the cyclical pathway ( F (9, 368) = 7.1, P < 0.001).